ISRDB Cryoprotectant database for protein crystals

Number of Result: 1777. [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] [33] [34] [35] [36] [37] [38] [39] [40] [41] [42] [43] [44] [45] [46] [47] [48] [49] [50] [51] [52] [53] [54] [55] [56] [57] [58] [59] [60]

Protein Name	Crystallization Method	Freezing Method
(1R;6R)-2-succinyl-6-hydroxy-2;4-cyclohexadiene-1-carboxylate (SHCHC) synthase	hanging-drop vapour-diffusion	A crystal was cryoprotected by a gradual increase of ethylene glycol up to 15% in 3% steps and transferred frozen to the nitrogen-gas stream (100K).
(S)-2-hydroxypropylphosphonic acid epoxidase	vapour-diffusion	The crystal was picked up in a 0.05x0.05 mm loop; flash-frozen to 105K in a cold nitrogen-gas stream and subjected to X-ray diffraction.
(cytosine-5)-DNA methyltransferase NlaX	sitting-drop vapour-diffusion	Crystals were soaked in the crystallization buffer with substrate and 30% glycerol as a cryoprotectant for 15 min and were then cooled in a nitrogen stream at 100K.
(nucleoside-2'-O-)-methyltransferase	vapour-diffusion	Diffraction data were collected from a flash-cooled crystal suspended in the crystallization mother liquor supplemented with 20% glycerol for cryoprotection.
(nucleoside-2'-O-)-methyltransferase	vapour-diffusion	The crystals were cryoprotected by transfer to their mother liquor supplemented with 20% glycerol prior to flash-cooling in liquid nitrogen.
1-L-myo-inositol 1-phosphate synthase		a single crystal was transferred to cryoprotectant stabilizing solution (5% PEG 8000; 0.1M sodium acetate pH 4.5; 30% glycerol) and flash-frozen
12S central subunit of transcarboxylase (free)	sitting-drop vapour-diffusion	crystals could be mounted directly from the crystallization drop as the mother liquor was an effective cryoprotectant
12S central subunit of transcarboxylase (substrate-bound)	sitting-drop vapour-diffusion	crystals could be mounted directly from the crystallization drop as the mother liquor was an effective cryoprotectant
1;3-1;4-beta-D-glucanase	hanging-drop vapour-diffusion	crystals were soaked in cryoprotectant consisting of 10% glycerol in the reservoir solution for 1 min
1;3-1;4-beta-D-glucanase	hanging-drop vapour-diffusion	crystals were soaked in cryoprotectant consisting of 10% glycerol in the reservoir solution for 1 min
1;3-propanediol dehydrogenase	sitting-drop vapour diffusion	Appropriate cryoprotecting conditions were obtained by soaking crystals for 1 min in crystallization solution with 25%(v/v) glycerol.
1;4-beta-D-glucan glucohydrolase	hanging-drop vapour-diffusion	Crystal freezing included a 3 min soaking of the crystal in a cryoprotectant solution containing the original crystallization solution and 20% glycerol. The pre-soaked crystal was then submitted to immediate flash-freezing directly within a cold nitrogen-gas stream.
1H-3-hydroxy-4-oxoquinaldine 2;4 dioxygenase	hanging-drop vapor-diffusion	Crystals grown from 1.65 M sodium/potassium tartrate; 0.1 M HEPES pH 7.0 and 30 mM CuCl2 were transferred for a few seconds into a reservoir containing 1.7 M sodium/potassium tartrate; 0.1 M HEPES pH 7.0; 30 mM CuCl2 and 15%(v/v) glycerol and vitrified in liquid nitrogen for data collection. Although the high salt concentration present in the reservoir allows a high degree of cryoprotection; we observed improved behaviour in the presence of glycerol.
1H-3-hydroxy-4-oxoquinoline 2;4 dioxygenase	hanging-drop vapor-diffusion	Crystals were prepared for freezing by transferring them into artificial mother liquor containing increasing concentrations of sodium formate. At each step; the sodium formate concentration was increased by 0.25 M and the crystals were equilibrated in the solutions for 3 min each. The maximum concentration of sodium formate used was 6 M. Crystals were flash-cooled by transferring them into liquid nitrogen. All data were collected at 100 K.
2'-5' RNA ligase	hanging-drop vapor-diffusion	A single crystal was mounted in a loop and flashcooled in a stream of nitrogen gas after soaking in mother liquor containing 15% glycerol.
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	vapour-diffusion	A crystal was cryopreserved by soaking in substituted mother liquor (0.2M ammonium sulfate; 0.1M sodium acetate; 25% PEG 4000 and 18% 2-methyl-2;4-pentanediol) for 10s and then maintained at 100K in a stream of nitrogen gas.
2-dehydro-3-deoxygalactarate aldolase	hanging-drop vapour-diffusion	Prior to data collection; crystals were cryoprotected in a buffer consisting of 20% glycerol added to the mother liquor and were then flash-cooled in liquid nitrogen.
2-deoxy-scyllo-inosose synthase	hanging-drop vapour-diffusion	Prior to flash-freezing; the crystals were briefly soaked in a cryoprotectant solution containing 20%(v/v) glycerol; 35%(w/v) PEG 8000; 0.6M NaCl and 0.1M MES buffer pH 6.0.
2-enoyl-CoA hydratase 2 domain	hanging-drop vapour-diffusion	The crystals were soaked in cryoprotecting mother liquor and flash-frozen in a nylon CryoLoop in a cold nitrogen stream before data collection. Crystals were incubated for 10min in cryosolution(23% ethylene glycol; 12% PEG 4000; 0.1M HEPES pH 7.0).
2-enoyl-CoA hydratase 2 domain (SeMet)	hanging-drop vapour-diffusion	The crystals were soaked in cryoprotecting mother liquor(20% ethylene glycol; 14% PEG 4000; 0.1M HEPES pH 7.0) and flash-frozen in a nylon CryoLoop in a cold nitrogen stream before data collection. The crystal was incubated for 5min before flash-freezing.
2-hydroxybiphenyl 3-monooxygenase	hanging-drop vapour-diffusion	the crystals were transferred directly into cryoprotectant solution consisting of 30%(v/v) glycerol; 1.6M ammonioum sulfate; 0.1M NaCl; 0.1M MES-NaOH pH 7.5
2-keto-3-deoxygluconate kinase	sitting-drop vapour-diffusion	For data collection; the crystals were mounted in nylon-fibre loops and flash-cooled in a dry nitrogen stream at 100K.
2-keto-3-deoxygluconate kinase-ATP	sitting-drop vapour-diffusion	In order to avoid the interference of ammonium sulfate; it was gradually replaced by magnesium chloride and crystals were kept in this solution for 1h prior to cryoprotection. The cryoprotectant contained 33%(v/v) ethylene glycol in addition to magnesium chloride; ligands and buffer. For data collection; the crystals were mounted in nylon -fibre loops and flash-cooled in a dry nitrogen stream at 100K.
2-methyl-3-hydroxypyridine- 5-carboxylic acid (MHPC) oxygenase	hanging-drop vapour-diffusion	The crystals were transferred into 20% PEG 400; 100mM sodium acetate trihydrate buffer pH 5.0 prior to flash-freezing in liquid nitrogen.
2-oxo-hept-4-ene-1;7-dioate hydratase (HpcG)	sitting-drop vapour-diffusion	Crystals were picked up from the crystallization drops; soaked in mother liquor containing 30% glycerol for 30 s; transferred to a cryostream and cooled to 100K.
20K endoglucanase	hanging-drop vapour-diffusion	crystals were harvested with stepwise transfer via a series of solutions containing increasing concentrations of the cryoprotective agent (10; 20 and 30% glycerol) -->crystals were flash-frozen in a cold stream at 120K.
20alpha-hydroxysteroid dehydrogenases	hanging-drop vapour-diffusion	incubated for a few days in crystallization buffer to which a cryoprotectant agent(12% ethylene glycol); the steroid(freshly dissolved in 100% ethylene glycol); and cofactor had been added -> flash-frozen in a nitrogen-gas stream
2;4-dihydroxy-hepta-2-ene-1;7-dioate aldolase (HpcH)	sitting-drop vapour-diffusion	To avoid freezing; crystals were soaked in a cryoprotectant solution containing 75% mother liquor and 25% glycerol.
2C-methyl-D-erythritol-2;4-cyclodiphosphate synthase	hanging-drop vapour-diffusion	Crystals were cryoprotected using reservoir solution supplemented with 10%(v/v) glycerol and flash-cooled by rapidly moving them into a cold nitrogen stream.
3'-phosphoadenosine-5'-phosphosulfate synthetase 1	hanging-drop vapour-diffusion	The crystals were transferred to cryosolution (the crystallization solution containing 30% glycerol) in five steps and frozen in liquid nitrogen.